PHYTOCHEMICAL AND ANTI-INFLAMMATORY STUDIES ON THE HEXANE EXTRACT OF THE STEM BARK OF STEGANOTAENIA ARALIACEAHOSCHTS (APIACEAE)
- Project Research
- 1-5 Chapters
- Abstract : Available
- Table of Content: Available
- Reference Style: APA
- Recommended for : Student Researchers
- NGN 3000
ABSTRACT
Inflammation has been implicated in virtually all human and animal diseases. It has become the focus of global scientific research, more so, since the currently used anti-inflammatory agents both steroidal and non-steroidal are prone to evoking serious adverse reactions. Steganataeniaaraliacea(Apiaceae)stem bark has been reported to be used in traditional medicine for the treatment of asthma and rheumatism in East Africa. In this study, thehexane extract of the stem bark of the plant was evaluated for its anti-inflammatory property and with a view to isolating bioactive compounds from it.Methods for evaluating macroscopical features and physico-chemical properties were used for the pharmacognostic studies on the stem bark, chromatographic techniques including the thin layer chromatography (TLC) and column chromatography using silica gel were used for the phytochemical studies on the hexane extract of the plant while the anti-inflammatory study was also carried out on the hexane extractusing formalin induced paw oedema in rats. The macroscopical features of the whole stem bark was described as yellowish-green in colour, waxy and short fractured with an aromatic odour while the powdered stem bark was described as brown in colour, aromatic in odour with a sweet bitter taste. The physico-chemical properties of the bark determined include total ash value 10.67%, acid-insoluble ash value 4.00%, water-soluble ash value 1.25%, moisture content 8%, watersoluble extractive value 2.87%, alcohol-soluble extractive value 6.67% and petroleum ethersoluble extractive value 1.27%. The TLC profiling of the hexane extract of the bark showed the presence of steroids/terpenes. The column chromatography of the hexane extract of the stem bark lead to isolation of Compound UF which was characterized using the 1H-NMR spectroscopy and its physical properties. Compound UF was identified as Hentriacotane (C31H64) based on the available spectroscopic data and physical properties which agreed well with those reported in the viii literature. The acute toxicity studies of the hexane extract revealed (LD50) of 282.84mg/kg suggesting the extract is moderately toxic. The effect of the hexane extract on formalin induced paw oedema in rats revealed a dose dependant inhibition of the paw oedema when compared to the reference. The concentrations of the extract at 35, 70 and 140 mg/kg of the extract showed 41%, 42% and 50% inhibition respectively at the peak of the paw oedema which were statistically significant at P<0.05. This indicates a dose dependent anti-inflammatory activity of the extract.
Related Project Materials
IMPACT OF PRICING AND MARKETING OF BEVERAGE
BACKGROUND OF THE STUDY
Decision about prices of a company’s product and services are among the most important ac...
ETHICS AND ACCOUNTABILITY IN THE NIGERIAN CIVIL SERVICE: A CASE OF FEDERAL INLAND REVENUE SERVICE, ENUGU (2000-2010)
ABSTRACT
This study interrogates the root causes of persistent unethical comportment, erosion of Values, and poor accoun...
AN ASSESSMENT OF THE IMPLEMENTATION OF UNIVERSAL BASIC EDUCATION IN NIGERIA
ABSTRACT
The focus of this study is on the assessment of the implementation of various aspects of the UBE pro...
IMPACT OF ADULT LITERACY PROGRAMS ON RURAL DEVELOPMENT
Abstract: The impact of adult literacy programs on rural development is a critical area of study in understanding the socio-economic advancements w...
THE USE OF INFORMATION COMMUNICATION TECHNOLOGIES IN PUBLIC RELATIONS PRACTICE
EXCERPT FROM THE STUDY
In order to put the contemporary practice and relevance of PR in proper perspective, it is import...
ECONOMIC ANALYSIS OF LAYER PRODUCTIONS
INTRODUCTION
One of the major challenges facing most developing countries is the satisfaction of the ev...
PROSPECTS AND PROBLEMS OF E-MARKETING IN THE HOSPITALITY INDUSTRY
BACKGROUND OF THE STUDY
According to the definition provided by the American Marketing Association (200...
THE ROLE OF COMPUTER IN FRAUD DETECTION AND PREVENTION
BACKGROUND OF THE STUDY
Since 1986 when structural adjustment programme was introduced, the banking bus...
THE EFFECT OF LABORATORY METHOD OF TEACHING MATHEMATICS ON THE ACHIEVEMENT OF JSS2 STUDENTS
Abstract
The study focused on the effect of the laboratory method of teaching on the achievement of J.SS II students in...
THE INFLUENCE OF ENDSARS PROTEST ON POLITICAL INTEREST OF NIGERIA YOUTH
Background of the Study
The Special Anti-Robbery Squad, or SARS, is a special police team that was esta...
